Coelenterazine h  (Synonyms: 腔肠素h; 2-Deoxycoelenterazine; CLZN-h)

Coelenterazine h (2-Deoxycoelenterazine), a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h is more sensitive to Ca²⁺, thus providing a valuable tool for measuring small changes in Ca²⁺ concentrations^[1][2][3][4].

Coelenterazine h (1-10 μM) 可用作 RLuc8 的发光底物^[4]。
在 BRAC 的 Ca²⁺ 结合动力学测量中，将 5 nM BRAC 蛋白与 20 μM Coelenterazine h 在不同浓度的 Ca²⁺ 缓冲液中快速混合后，在 1 kHz 下监测 BRAC 的 Venus (530 nm) 发射强度^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

407.46

C₂₆H₂₁N₃O₂

50909-86-9

固体

Yellow to brown

腔肠素h

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

• [1]. Jiang T, et al. New bioluminescent coelenterazine derivatives with various C-6 substitutions. Org Biomol Chem. 2017 Aug 23;15(33):7008-7018.  [Content Brief]

  [2]. M R Knight, et al. Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins. J Cell Biol. 1993 Apr;121(1):83-90.  [Content Brief]

  [3]. Kazushi Suzuki, et al. Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat Commun. 2016 Dec 14:7:13718.  [Content Brief]

  [4]. Kenta Saito, et al. Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. PLoS One. 2010 Apr 1;5(4):e9935.  [Content Brief]